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KMID : 0923620160160040249
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Immune Network
2016 Volume.16 No. 4 p.249 ~ p.255
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Dependence of RIG-I Nucleic Acid-Binding and ATP Hydrolysis on Activation of Type I Interferon Response
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Baek Yu-Mi
Yoon Soo-jin
Hwang Yeo-Eun
Kim Dong-Eun
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Abstract
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Exogenous nucleic acids induce an innate immune response in mammalian host cells through activation of the retinoic acid-inducible gene I (RIG-I). We evaluated RIG-I protein for RNA binding and ATPase stimulation with RNA ligands to investigate the correlation with the extent of immune response through RIG-I activation in cells. RIG-I protein favored blunt-ended, double-stranded RNA (dsRNA) ligands over sticky-ended dsRNA. Moreover, the presence of the 5’-triphosphate (5’-ppp) moiety in dsRNA further enhanced binding affinity to RIG-I. Two structural motifs in RNA, blunt ends in dsRNA and 5’-ppp, stimulated the ATP hydrolysis activity of RIG-I. These structural motifs also strongly induced IFN expression as an innate immune response in cells. Therefore, we suggest that IFN induction through RIG-I activation is mainly determined by structural motifs in dsRNA that increase its affinity for RIG-I protein and stimulate ATPase activity in RIG-I.
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KEYWORD
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Retinoic acid inducible gene I (RIG-I), Interferon induction, 5’-terminal triphosphate, ATP hydrolysis, RNA binding
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